Colony Surveillance Assay™(CSA): Simian TB Test provides a sensitive, reliable, and affordable tool to supplement your TB surveillance program.

The CSA: Simian TB Serology Test identifies animals previously exposed to M. tuberculosis by screening for serum antibodies specific to M. tuberculosis. Current requirements rely on the Tuberculin Skin Test (TST) to be performed, but can often miss latent TB infections that can compromise your colony health.

The CSA: Simian TB serological test screens for the presence of TB-specific antibodies (IgG) in serum from nonhuman primates (NHPs). This test uses a panel of 4 peptides representing 3 proteins found in the RD (region of difference) from tuberculous Mycobacteria.  including: M. tuberculosis, M. kansasii, and M. bovis. It does not distinguish between these species, but shows that the sample tested contains antibodies specific to epitopes from TB. These peptides are not present in M. avium and should exclude M. avium – specific antibodies.

CSA: Simian TB Assay

The CSA: Simian TB assay uses the AIM platform. Our proprietary TB-specific antigens (1) are immobilized on the bottom of a 96-well plate. Serum is added to one well, and antibodies to controls and TB-antigens bind to the surface. Immune complexes are detected with an anti-simian IgG gold conjugate antibody. Signal is enhanced with SilverQuant reagents, to result in a stabile grey signal.

In each well of an assay plate, control or test antigens are arrayed into a specific pattern in the bottom of the plate. For each sample tested, a 1:400 dilution of serum is added to each well. Four replicate measurements are captured for each sample, and up to 94 samples can be run in the same plate.

Recommended Use

While the TST the only required test for imported NHPs, it is known that latent or subclinical infections with M. tuberculosis can be missed by the TST (2-7) . Moreover, animals can become sensitized to repeated TST over time (8). We recommend using the CSA: Simian TB test to supplement the required TST testing regimen. For importing animals, this can begin while the animal is in quarantine. For animals entering closed colonies but originating in the U.S., testing can be performed prior to transport. While an individual test result may help identify a latently infected animal, the best use of this test is to routinely monitor all animals to be able to compare to baseline values for the individual animal and for the colony itself.

Questions?

Please contact Dr. Kimberly Luke to discuss your testing needs. 

More information on packing requirements, shipping address, shipping methods, and sample submission forms can be found here.

Citations

  1. US Patent No. 9,404,923 “Mycobacterium tuberculosis specific peptides for detection of infection or   immunization in non-human primates.”
  2. Garcia, M. A., et al., Outbreak of Mycobacterium bovis in a conditioned colony of rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques. Comp Med, 2004. 54(5): p. 578-84.
  3. Mayhall, C. G., V. A. Lamb, and P. H. Coleman, Infection in rhesus (Macaca mulatta) and squirrel (Saimiri sciureus) monkeys due to Mycobacterium tuberculosis phage type B. Outbreak in a primate colony. J Med Primatol, 1981. 10(6): p. 302-11.
  4. Panarella, M. L. and R. S. Bimes, A naturally occurring outbreak of tuberculosis in a group of imported cynomolgus monkeys (Macaca fascicularis). J Am Assoc Lab Anim Sci, 2010. 49(2): p. 221-5.
  5. Payne, K. S., et al., Mycobacterium tuberculosis infection in a closed colony of rhesus macaques (Macaca mulatta). J Am Assoc Lab Anim Sci, 2011. 50(1): p. 105-8.
  6. Ward, G. S., et al., Use of streptomycin and isoniazid during a tuberculosis epizootic in a rhesus and cynomolgus breeding colony. Lab Anim Sci, 1985. 35(4): p. 395-9.
  7. Zumpe, D., M. S. Silberman, and R. P. Michael, Unusual outbreak of tuberculosis due to Mycobacterium bovis in a closed colony of rhesus monkeys (Macaca mulatta). Lab Anim Sci, 1980. 30(2 Pt 1): p. 237-40.
  8. Snyder, S. B. and J. G. Fox, Tuberculin testing in rhesus monkeys (Macaca mulatta): a comparative study using experimentally sensitized animals. Lab Anim Sci, 1973. 23(4): p. 515-21.
  9. Bushmitz, M., et al., Guidelines for the prevention and control of tuberculosis in nonhuman primates: recommendations of the European Primate Veterinary Association Working Group on Tuberculosis. J Med Primatol, 2009. 38(1): p. 59-69.